DNA Containing Hypochlorous Acid Was Activated by the Absolute Homeostasis Energy Source

Outline: 

Hypochlorous Acid is a weak acid and polyatomic ion oxidizer.
It is broadly used as the active sanitizer in laboratory; it is generated in activated neutrophils by myeloperoxidase-mediated peroxidation of chloride ions, and contributes to the destruction of bacteria. Myeloperoxidase (MPO) is the most abundantly expressed in neutrophils granulocytes (a subtype of white blood cells). MPO produces hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-) and uses to destroy or kills bacteria and other pathogens. If MPO is deficiency, the function of neutrophils to kill bacteria will be inhibited; it also decreases the resistant ability for organisms to be infected by microorganisms in the cells. The characteristics of hypochlorous acid are following:

1.	Addition of chlorine to water gives both hydrochloric acid and hypochlorous acid. Cl2 + H2O → HCl + HClO
2.	In the presence of light or transition metal oxides of copper, nickel, or cobalt accelerates the exothermic decomposition into hydrochloric acid and oxygen. 2Cl2 + 2H2O → 4HCl+O2
3.	HClO is a stronger oxidant than chlorine under standard conditions. 2HClO (aqu.) + 2H++ 2e－  Cl2 (g.) + 2H2O  E=+1.63v
4.	HClO is a sulfhydryl inhibitor, it can completely inactivate proteins containing sulfhydryl groups to form disulfide bonds. Hypochlorous acid reacts with amino acids that have amino group side-chains, with the chlorine from HClO displacing a hydrogen, resulting in an organic chloramines and amino acids rapidly decompose due to organic chloramines decayed by internal rearrangement and fewer available NH2 group promoted attack on the peptide bond, resulting in cleavage of protein. McKenna and Davies found that 10mM or greater HClO is necessary to fragment protein in vivo. Consistent with these results, the chloramines undergoes a molecular rearrangement, releasing HCl and ammonia to form an amide (-CONH2). This amide group can react with another amino group to form a Schiff base (>C=N-), causing cross-linkage and aggregation of proteins.
5.	HClO interferes with DNA base pairing and decreases in viscosity of DNA exposed to HClO similar to that seen with heat denaturalization. The sugar moieties are nonreactive and the DNA backbone is not broken. The cleavage of pyridine ring occurs when excess HClO is present.
6.	Hypochlorous acid reacts with unsaturated bonds in lipids, but not saturated bonds, and the OCl- ion does not participate in reactions. The reaction occurs by hydrolysis with addition of chlorine to one of the carbons and a hydroxyl to the other to form chlorhydrin. It will disrupt lipid bilayers and increase permeability.
7.	Escherichia coli exposed to hypochlorous acid lose viability due to inactivation of many vital systems. HClO has a reported LD50 of 0.0104 to 0.156ppm and 2.6 ppm caused 100% growth inhibition in 5 minutes.
8.	Inhibition of glucose oxidation is a major factor in the bactericidal nature of chlorine solutions. The active agent diffused across the cytoplasmic membrane to inactive key sulfhydryl-containing enzymes in the glycolytic pathway. HClO also blocks induction of β-galactosidase by added lactose.
9.	Exposure to HClO may affect ATP hydrolysis and proton co-transport, and inactivate transport protein resulting loss viability and block of nutrient uptake.
10.	Succinic hydrogenase was inhibited by HClO, which lead to disrupt of electron transport and stop the flow of electrons to oxygen, it can cause of bacterial inactivation.
11.	HClO destroys cytochrome, breaks iron-sulfur clusters, decreases oxygen uptake, and loses adenine nucleotides.
12.	HClO stops the oxidation of cytochrome and inhibites Ubiquinol oxidase activity. It will affect of respiration function.
13.	HClO will affect energy change:
	(1). Lost the ability to regulate adenylate pool. (the total concentration of ATP, ADP, and AMP in the cells)
	(2). Metabolite uptake was reduced 45% after exposure to HClO, because HClO causes intracellular ATP hydrolysis.
	(3). ATP synthetase was inhibited by HClO, it involved in loss of ability to regenerate ATP.
14.	HClO inhibits protein synthesis and proceeds to affect DNA synthesis and replication in bacteria and loss of viability. During bacterial genome replication, the origin of replication (oriC) binds to proteins that are associated with the cell membrane, the HClO treatment decreases the affinity of extracted membrane for oriC, and decreased affinity also parallels loss of viability.
15.	HClO is known to cause post-translational modification (PTM) to proteins, the notable ones being cysteine and methionine oxidation, special involving polypeptides or proteins in the cells, including mobility of electrophoresis, and oxidation and reduction etc.

Hypochlorous acid affects DNA similar to that with heat denaturalization. The sugar moieties are nonreactive and the DNA backbone is not broken, but it can change the mobility of electrophoresis and affects Rf value. The purpose of the experiment is to prove that Mr. Yuan Lin utilized new discovered Absolute Homeostasis Energy Source to activate DNA containing hypochlorous acid under no molecular weight change, no structure change, no shape change, at temperature 25℃, atmosphere 1.0 atm, pH =7.0, and isolated closed space, no catalyst, no biological active substances, no chemical substances, and no physical functional force to contact. The DNA containing hypochlorous acid can change its mobility and affects Rf value. The biological meaning of this experiment is that the Sensory Humans can utilize the Energy in Absolute Homeostasis to activate DNA mobility to promote the efficiency of sanitization of hypochlorous acid.

The data shows that different Rf values in three treatments with DNA (control, HClO, and HClO with HAES ) in electrophoresis gel.

This research paper is restricted by the rule of unpublished paper, only portion of the experimental outline is presented. The formal research report and the complete information of functional and technical and its practical application will be provided to the members of Chinese Association for the Human Evolution and specific responsible members of the specialized committees for the purpose of academic research for educational training whenever it is decrypted.

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