Ribulose-1, 5-bisphosphate carboxylase/oxygenase rubsico is the specific enzyme catalyzing this key reaction of CO2 fixation in the chloroplast of green plants. It can catalyze the reaction of either CO2  or, alternatively, O2  with RuBP (Garrett & Charles, 2013, p. 741).

Rubisco is a very abundant enzyme found in the chloroplast stroma. It makes up about 50% of the total soluble protein in chloroplasts. It is estimated that there is about 4 x 107 tons of rubisco in the world, and therefore it is probably one of the most abundant proteins in the biosphere. Rubisco catalyzes the conversion of CO2 to simple (reduced) organic compounds, a process called carbon dioxide fixation or carbon fixation, also known as carbon assimilation. Carbon dioxide is assimilated via a cyclic pathway, its key intermediates constantly regenerated. The pathway was elucidated in the early 1950s by Melvin Calvin and his colleagues, and is often called the Calvin cycle (Nelson & Cox, 2005, pp. 752 & 754).

Net synthesis of glucose from CO2  and H2O occurs in green plants through photosynthesis. And the capacity to generate net synthesis of glucose is the basic distinguishing feature among autotrophic, heterotrophic, and mixtrophic organisms. The earth has gone through 4.6 billion years of evolution and so far, except for plants or photosynthetic microorganisms, no animals can carry out photosynthesis.

Although many animals, including the human body, can link CO2  to organic acceptors via catalytic reactions, these reactions cannot bring about a net accumulation of organic carbon. For example, in the biosynthesis of fatty acids, CO2   is covalently attached to acetyl-CoA to form malonyl-CoA. Nevertheless, the “fixed CO2 ” will be released in the very next reaction. Therefore such kind of process is an endergonic reaction in one’s own body.

The New Human Line has one set of carbon fixation machinery in the body, which can synthesize glucose from water and CO2  released from oxygenation. This net synthesis of glucose was tested by Benedict’s reagent, spectrometer, high- pressure liquid chromatography, and nuclear magnetic resonance. The statistical analysis showed significant difference
between the control samples and the activated samples by the New Human Line（p=0.023）, which  proved the net systhesis of carbonhydrate/glucose through carbon fixation in the New Human Line (See Table 1 , Figures 1 & 2 for details).

Plants utilizes the unique enzymatic machinery and ribulose-1,5-bisphosphate carboxylase/oxygenase to catalyze the conversion of  CO2 to reductive organic compound. The New Human Line utilizes the newly-discovered Absolutely Constant Energy Source and new biological engineering techniques to control the free energy of reactants and products, generating a balance in the total amount