Introduction:

Aspergillus flavus is a fungus. It is also called a mold. It grows by producing thread like branching filaments known as hyphae. A network of hyphae known as the mycelium secretes enzymes that break down complex food sources. The resulting small molecules are absorbed by the mycelium to fuel additional fungal growth. When young, the conidia of A. flavus appear yellow green in color. As the fungus ages, the spores turn to be dark green (Aspergillus flavus, 2005).

Aspergillus flavus can be pathogenic on several plant and animal species. The fungus can infect seeds of corn, peanuts, cotton, and nut trees. Unlike most fungi, Aspergillus flavus is favored by hot and dry conditions. The optimum temperature for growth is 37℃, but the fungus readily grows between the temperatures of 25-42℃. Such a high temperature optimum contributes to its pathogenicity on humans (Aspergillus flavus, 2005).

In 1989, Mr. Yuan Lin discovered the Absolutely Constant Energy Source (ACES). He can utilize ACES to activate the genome responsible for inheritance, development and performance of body functions in the human body to control specific amino acid sequences and protein peptide chain formation processes, directing cell division, replication and new organelle formation. ACES was also applied to induce changes in the irreversible process of spontaneous biochemical reactions of carbohydrates, lipids, proteins, amino acids, enzymes, nucleic acids, vitamins, hormones, and trace elements. Mr. Yuan Lin thus had his body experience an unprecedented leap forward in human evolution, which has thoroughly changed the human biological form, structure, and habits inherited over billions of years, to become the first successfully-evolved New Human Line.

The purpose of this experiment was to prove that Mr. Yuan Lin, the first successfully-evolved New Human Line, can utilize the Absolutely Constant Energy Source and the new biological engineering techniques to activate Aspergillus flavus and  inhibit the hyphal growth of Aspergillus flavus as well as the production of aflatoxins so as to control the carcinogenic reactions induced by Aspergillus flavus without any change in its molecular weight, structural formula, and conformation, at 25℃,1.0 atm, and pH 7.0, in a confined and isolated space, and with no contacts with catalysts, biologically active substances, chemical substances, and physical action forces.

Results and Discussion:

Table 1.  Comparison of average growth of the mycelium of Aspergillus flavus in different treatments (Test 1) (02/05/2008)

Figure1.  Line graph of average growth of the mycelium of Aspergillus flavus in different treatments (02/05/2008)

Table 2. Comparison of average growth of the mycelium of Aspergillus flavus in different treatments (Test 2) (02/05/2008)

Figure 2.  Line graph of average growth of the mycelium of Aspergillus flavus in different treatments. (02/05/2008).

Table 3. Comparison of average growth of the mycelium of Aspergillus flavus in different treatments (Test 3) (02/08/2008)

Figure 3. Line graph of average growth of the mycelium of Aspergillus flavus in different treatments. (02/08/2008) .

From Table 1 and Figure 1 (Test 1), we can see that the diameter of the mycelium of the fungi treated by ACES was shorter than that of the other 3 treatments and the control sample. Table 2 and Figure 2 (Test 2) and Table 3 and Figure 3 (Test 3) showed the same result as that of Test 1.

From Test 1 and Test 2, we can see that the hot energy state treatment could stimulate the growth of the mycelium because Aspergillus flavus is favored by hot and dry conditions.

From the above experimental data, we can see that the strong base energy state treatment and the strong acid energy state treatment had little effect on the inhibition of the growth of the mycelium. From the statistical analysis, it is shown that there is significant difference between the control sample and the ACES treatment, the hot energy state treatment, the cold energy state treatment, the strong acid treatment respectively in Test 1.  Test 2 and Test 3 also showed significant difference between the control sample and the ACES treatment sample.

From the above experimental data, we can see that the use of ACES can inhibit the growth of the fungus and reduce the production of aflatoxins as represented by the experimental results in Test 1 showing that it inhibited mycelial growth by 46.7% (56 hours after treatment), in Test 2 showing inhibition of mycelial growth by 10% (43 hours after treatment) and Test 3 showing inhibition of mycelial growth by 1.3% (70 hours after treatment). Statistical analysis indicated a significant difference between the ACES treatment and the control sample.

The Linyuan Mechanics can explain these experimental results. In the unpublished experiments performed in February, 2008,  ACES  was applied to Aspergillus flavus samples for different treatment time (3, 6, and 9 minutes) to see its effect on the mycelial growth of the fungus. These unpublished data showed that, 48 hours after treatment, the mycelial growth was inhibited by 2.5% in the 9-minute ACES treatment sample and by 0.83% in the 3-minute ACES treatment sample.

Conclusion:

From the above tests, it showed that the diameter of the mycelium of the samples treated by the Absolutely Constant Energy Source was shorter than that of the other treatments and the control sample. The hot energy state released by the fission of the Absolutely Constant Energy Source instead stimulated the mycelial growth of the fungus because Aspergillus flavus isfavored by hot and dry conditions. It shows that the Absolutely Constant Energy Source can inhibit the mycelial growth and reduce the aflatoxin production.

The strong acid energy state and the strong base energy state released by the fission of the Absolutely Constant Energy Source had little effect on the mycelial growth of Aspergillus flavus.

From the statistical analysis, we can see that there is significant difference between the ACES treatment and the control sample in Test 1, Test 2, and Test 3. It proved that the Absolutely Constant Energy Source can inhibit the mycelial growth of Aspergillus flavus and reduce the aflatoxin production. We are indebted to further study on whether the Absolutely Constant Energy Source has the same capability of inhibition on those plants and animals including humans that are inflected by Aspergillus flavus.

References:

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