Liza Chen, Ying-Tung Lin, and Yuan Lin |
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Introduction: Genomic DNA is a very long molecule, which must be fitted into a small space inside a cell or virus particle. DNA condensation is the collapse of extended DNA chains into compact, orderly particles containing only one or a few molecules (Bloomfield, 1997, p.2). DNA condensation has become a lively area for research in diverse areas of science. In biochemistry, biophysics, and molecular biology, it represents a process by which the genetic information is packaged and protected. And in biotechnology and medicine, it provides a promising means whereby DNA containing genes of therapeutic interest can be prepared for transfer from solution to target cells for gene therapy applications (Bloomfield, 1997, p.2). Condensing agents generally work either by decreasing repulsions between DNA segments (e.g. neutralizing of phosphate charge, and/or reorienting water dipoles near DNA surfaces, by multivalent cations) or by making DNA-solvent interactions less favorable (e.g. by adding another polymer). Multivalent cations may also cause localized bending or distortion of the DNA, which can also facilitate condensation (Bloomfield, 1997, p.3). Multivalent cations bind electrostatically near the DNA surface, and cause DNA condensation by their correlated fluctuations and effects on water polarization. Though they have no other impact on DNA structure, they can profoundly distort the structure of DNA by slowly modifying its local structure (Bloomfield, 1997, pp.16-17). The purpose of this experiment is to prove that Mr. Yuan Lin, the first successfully-evolved New Human Line, can utilize the Absolutely Constant Energy Source (ACES) and the new biological engineering techniques to copy and constitute multivalent cations (Fe+++) with energy through resonance, then insert them into DNA to affect DNA condensation, and also check the efficiency of their passing through a 3.8 cm thick steel plate barrier without any change in the molecular weight, structural formula, and conformation, at 25℃,1.0 atm., and pH 7.0, in a confined and isolated space, with no contact with catalysts, biologically active substances, chemical substances, and physical action forces.
Results and Discussion: From the results of the electrophoresis, we can see that for the DNA samples which were inserted with the energy state of FeCl3 copied and constituted with energy through resonance by Mr. Yuan Lin who utilized ACES to perform this treatment, their Rf values are lower than those of the control groups. In Experiment 1.1, the Rf value is 0.404 for the treated sample, but is 0.418 for the control sample (Figure 1, Table 1). And in Experiment 1.2, the Rf value is 0.430 for the treated sample, but is 0.438 for the control sample (Figure 2, Table 1).
Table 1. Comparison of the Rf values between two different treatments in Experiments 1.1 & 1.2 In the second experiment, Mr. Yuan Lin utilized ACES to copy and constitute the energy state of FeCl3 with energy through resonance, and then have the energy state pass through a 3.8 cm steel plate barrier to be inserted into the DNA samples. From the experimental results, we can see that for both the DNA samples which were inserted with the energy state of FeCl3 copied and constituted with energy through resonance and those which were inserted with the energy state of FeCl3 that had passed through a 3.8 cm steel plate barrier by Mr. Yuan Lin who utilized ACES to perform these treatments, their Rf values are lower than those of the control group. In Experiment 2.1, the Rf value is 0.390 for the first two treatments, but is 0.404 for the control group (Figure 3, Table 2). In Experiment 2.2, the Rf value is 0.389 for the first treatment, 0.394 for the second treatment, but is 0.403 for the control group (Figure 4, Table 2).
Table 2. Comparison of the Rf values among three different treatments in Experiments 2.1 & 2.2 
From the experimental results, we can see that the Absolutely Constant Energy Source is not affected by time, space, and other substances because the Rf values of the first two treatments (inserted with the copied Fe+++ energy state and with the copied Fe+++ energy state passing through the steel plate barrier) are lower than those of the control group. The data were very similar. The results also show that FeCl3 affects the mobility rate of DNA because Fe+++ will bind to the negatively charged DNA phosphate and partially neutralize the negative charge on DNA. If the concentration of multivalent cations is increased, it will lead to DNA condensation, or even aggregation. This mainly results from the mutual electrostatic attraction between DNA and cations. When metal ions bind to phosphate groups, it will lead to a lower mobility for the migrating radical cation in the condensate, and cause subtle migration of DNA on the absorption spectrum. Das and Schuster (2005) reported that the formation of DNA condensates with the multivalent cation inhibits the migration of cations due to the decreased probability for forming the charge-transfer-effective states (p.14231). From the above experimental results, the Absolutely Constant Energy Source can be utilized to copy and constitute multivalent cations with energy through resonance to affect DNA condensation.
From the results of electrophoresis, we can see that for both the DNA samples inserted with the energy state of FeCl3 copied and constituted with energy through resonance and those inserted with the energy state of FeCl3 that had passed through a 3.8 cm steel plate barrier by Mr. Yuan Lin who utilized ACES to perform these treatments, their Rf values are lower than those of the control group. This is mainly attributed to the fact that Mr. Yuan Lin increased the concentration of multivalent cations to induce stronger effects of DNA condensation. It can prove that Mr. Yuan Lin, the New Human Line, can utilize the Absolutely Constant Energy Source and the new biological engineering techniques to constitute multivalent cations with energy to affect DNA condensation.
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