Inhibitiion of Molds Liza Chen, Ying-Tung Lin, and Yuan Lin |
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Introduction: Mold is a kind of eukaryotic organism which belongs to the multicellular category. It is also one kind of microorganism. Microorganisms can be classified by their particle size and properties into five main categories: Viruses, Bacteria, Archea, Protists, and Fungi (molds). According to the current global literature, over one hundred thousand species of molds have already been found. Molds are heterotrophic organisms that have no sharp distinction between roots, stems, and pipes. They absorb other substances to sustain themselves. They are composed of single hyphae which then aggregate into a dense mycelium. This mycelium grows very fast because proteins and other synthesized substances are transported through the cytoplasm to the tips of the growing hyphae. Molds play a role in purifying the Earth’s ecological environment and also have specific functions in the manufacture of pharmaceutical products and fermented foods, through which they benefit humans a lot. On the other hand, molds easily spoil food, make trees rotten, evade the plant body, damage houses and buildings and then affect their exterior appearance. The human body is more likely to be infected and thus becomes susceptible to diseases or allergies. The purpose of this experiment is to prove that Mr. Yuan Lin, the first successfully-evolved New Human Line, can utilize the Absolutely Constant Energy Source (ACES) and the new biological engineering techniques to inhibit the growth of mold hyphae and the development of mold spores so as to extend the life of molds without any change in their molecular weight, structural formula, and conformation, at 25℃, 1.0 atm, and pH 7.0, in a confined and isolated space, and with no contact with catalysts, biologically active substances, chemical substances, and physical action forces. Figure 1. The molds growing from the seeds of Impatiens balsamina in agar.
The first experiment: Table 1. Comparison of the diameter of mycelial growth between two treatments in Experiment 1(20080121)
Statistical analysis:
The second experiment: Table 2. Comparison of the diameter of mycelial growth between the ACES-treatments and the control in Experiment 2(20080125)
Statistical analysis:
The third experiment: Table 3. Comparison of the diameter of mycelia growth between the ACES-treatments and the control in Experiment 3 (20080128)
Statistical analysis: From Tables 1 and 2, and Figures 1 and 2 ( for the first and second experiments), we can see that the mycelium samples exhibit parallel growth in the control group and the experiment group, but the diameters of the ACES-treated mycelium samples are smaller than those of the control samples. In the first experiment, the mycelia stayed in a static condition from the 42nd hour after being treated with ACES to the 50.5th hour. The static condition lasts for 8 hours 30 minutes in total. In the second experiment, the static condition lasts for 5 hours. From Table 3 and Figure 3, we can see similar results as the first and second experiments. The diameters of the ACES-treated mycelium samples are smaller than those of the control samples. And the mycelia stayed in a static condition from the 44th to 49th hour after treatment. The static condition lasts for about 5 hours in total. Thus we can see that ACES has an inhibitory effect on the growth of mold mycelia and the development of mold spores (molds are made up of multiple cells).
From the tables and figures, we can see that all the growth rates of the mycelia treated with ACES appear to be slower than those of the control groups because the mycelia and spores are inhibited by ACES (p=0.000). In the first experiment, the mycelia can stay in a static condition for about 8 hours 30 minutes. In the second experiment, they can stay in a static condition for about 5 hours. It can prove that Mr. Yuan Lin, the New Human Line, can utilize the Absolutely Constant Energy Source and the new biological engineering techniques to regulate the growth of mold mycelia and the development of the spores so as to significantly prolong the life of molds.
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