Liza Chen, Ying-Tung Lin, and Yuan Lin |
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Introduction: Rapeseed (Brassica napus) belongs to the genus Brassica in the family Brassicaceae (mustard or cabbage family). It is closely related to broccoli, cabbage, and cauliflower. The name derives from the Latin for turnip, rāpa or rāpum. Rapeseed can be used as fertilizer, pig feed, etc. Rapeseed (Canola) oil is a good cooking oil as it contains both omega-6 and omega-3 fatty acids in a ratio of 2:1. Its omega-3 fat content is only next to that in flaxseed oil. It can be easily digested and absorbed by the human body and has the function of reducing the body’s serum cholesterol. The triglyceride it contains also can soften blood vessels and prevent thrombosis, and has a particularly significant effect on fat metabolism in the human body. Rapeseed is a type of highly nutritive vegetables. Its protein content is 0.7 times higher than that in cabbage, vitamin A content 60 times higher, vitamin B1 content twice higher, vitamin B2 content three times higher, vitamin C content twice higher, calcium and iron content five times higher than that in cabbage. Its nutrient values are shown in Table 1.
Table 1. Nutrient values of rapeseed
Note. “-” means undetected. Data adapted and translated from https://consumer.fda.gov.tw/FoodAnalysis/ingredients.htm
Rapeseed can be grown in both winter and spring. The optimal germination temperature is between 20°C and 25°C, and the suitable temperature for growth is between 15°C and 20°C. It has strong adaptive capability to adjust to a variety of environments, and doesn’t have critical requirement for the choice of soil as long as the pH value ranges between 5.0 and 8.0. With good drainage, the soil will be more suitable for growth. The purpose of this experiment is to prove that Mr. Yuan Lin, the first successfully-evolved New Human Line, can utilize the Absolutely Constant Energy Source (ACES) and the new biological engineering techniques to activate the growth of rapeseed at different temperatures to affect its germination rate without any change in its molecular weight, structural formula, and conformation, at 25℃, 1.0 atm, and pH 7.0, in a confined and isolated space, and with no contact with catalysts, biologically active substances, chemical substances, and physical action forces.
Results & Discussion:
Table 2. The seed germination rate at constant 25°C
Table 3. The germination rate of seeds at constant 30°C
Table 5. The seed germination rate at constant 22.5°C
Table 6. Results of statistical analysis
From Table 2 and Figure 1, we can see that the seed germination rates of the non-treatment (control) samples are higher than those of the hot-energy-state treatment and the cold-energy-state treatment samples. They are 96.7%, 92.0%, and 92.0%, respectively. This is due to the fact that the optimal temperature for rapeseed germination is between 20°C and 25°C so that both the hot-energy-state treatment and the cold-energy-state treatment will affect the seed germination rate. From Table 3 and Figure 2, we can see that the results of seed germination are similar to those shown in Table 2 in that the seed germination rates for the control group appear to be higher than those for the other two treated samples and that the hot-energy-state treatment samples exhibit the lowest germination rates because the seeds were incubated at 30°C, which has exceeded the temperature range suitable for seed germination. From Table 4 and Figure 3, we can see that the seeds treated with cold energy state show the best germination rates probably because the seeds broke the dormancy and thus increase the germination rates. From Table 5 and Figure 4, it can be seen that there is no significant difference among these three treatments because all the seeds were incubated at 22.5°C, which is within the suitable temperature range for seed germination and thus leads to no significant difference among them. From the results of statistical analysis (ANOVA), we can see significant differences in these three factors: treatment with different energy states, temperature, and time. The samples treated with ACES exhibit correlation between the seed germination rate and the temperature, treatment techniques, seed properties and growth stages.
From the tables, the figures, and the results of statistical analysis (ANOVA) above, we can find that the seed germination rate relates to the temperature, treatment techniques (hot-energy-state treatment, cold-energy-state treatment, and non-treatment), seed properties and growth stages. It can prove that Mr. Yuan Lin, the New Human Line, who can utilize the Absolutely Constant Energy Source and the new biological engineering techniques to regulate the germination of rapeseed.
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