Introduction:

Candida albicans is a diploid fungus (one kind of yeast) which is capable of mating but incapable of meiosis. It will cause oral and genital infections in the human body. To infect host tissue, the usual unicellular yeast-like form of C. albicans will switch into an invasive, multicellular filamentous form.

Glucose is the principal carbon and energy source for nearly all cells. Glucose responsiveness and sensitivity have a regulatory effect in many organisms. Glucose is very important for hexose transport in yeast. Saccharomyces cerevisiae, for example, has at least 17 different hexose transporters to transport glucose under different conditions; C. albicans has at least 20 kinds of S. cerevisiae and therefore it requires a large amount of hexose transporters to ferment glucose. And as it can produce only two molecules of ATP in metabolism, it requires sufficient glucose to provide energy. Usually C. albicans prefers to metabolize glucose by respiration because in this way it can gain 38 ATP molecules per oxidized glucose molecule and in the meanwhile produce many hexose transporters. It can be said that C. albicans requires a variety of carbohydrates as a source of carbon to flourish and grow.

The purpose of this experiment is to prove that Mr. Yuan Lin, the first successfully-evolved New Human Line, can utilize the Absolutely Constant Energy Source (ACES) and the new biological engineering techniques to enhance thegrowth and spore formation of C. albicans and extend its life without any change in its molecular weight, structural formula, and conformation, at 25℃, 1.0 atm, and pH 7.0, in a confined and isolated space, and with no contact with catalysts, biologically active substances, chemical substances, and physical action forces.

Results & Discussion:

Figure 1. Comparison of the diameter of the mycelium between two treatments.

Statistical analysis:

Figure 2. Comparison of the diameter of the mycelium between two treatments.

Statistical analysis:

From the experimental results, we can see that the samples treated with the energy state of glucose constituted and duplicated by Mr. Yuan Lin with ACES display greater diameters of the growing mycelium than the control group. This is due to the fact that glucose is the source of carbon and energy for fungi and that the energy state of glucose constituted and duplicated by Mr. Yuan Lin with ACES significantly enhances the growth and spore formation of C. albicans and thus extend its life.

Conclusion:

From the above experiment results, we can see that the samples treated with the energy state of glucose constituted and duplicated by Mr. Yuan Lin with ACES display greater diameters of the growing mycelium than the control group. The statistical analysis shows a significant difference (p=0.000). It proved that Mr. Yuan Lin, the New Human Line, can utilize the Absolutely Constant Energy Source (ACES) and the new biological engineering techniques to prolong the life of Candida albicans.

References:

1. Candida albicans. (2018). Retrieved from https://en.wikipedia.org/wiki/Candida_albicans
2. Hubele, N. F., Runger, G. C., & Montgomery, D. C. (1998). Engineering statistics. New York, NY: John Wiley & Sons.
3. Mayer, F.L., Wilson, D.,& Hube, B. (2013). Candida albicans pathogenicity mechanism. Virulence 4(2):119-128. doi:10.4161/viru.22913
4. Michael, A. (2007). C. albicans, does it really have a sweet tooth? Retrieved from https://www.clinicaleducation.org/resources/reviews/c-albicans-does-it-really-have-a-sweet-tooth/